PageRuler™ Prestained Protein Ladder, 10 to 180 kDa

描述

Thermo Scientific PageRuler Prestained Protein Ladder is a mixture of 10 blue-, orange-, and green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.


Applications
• Monitoring protein migration during SDS-polyacrylamide gel electrophoresis
• Monitoring protein transfer onto membranes after western blotting
• Sizing of proteins on SDS-polyacrylamide gels and western blots
 

For Research Use Only. Not for use in diagnostic procedures.

Migration patterns of the PageRuler Prestained Protein Ladder in different electrophoretic conditions
The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. Migration patterns were determined using commercial precast mini gels.

SDS-PAGE band profile of the PageRuler Prestained Protein Ladder
Images are from a 4-20% Tris-glycine gel (SDS-PAGE) and subsequent transfer to membrane.

规格

内容及储存

Contents: 10 vials of 250 µL each
Storage buffer: 62.5 mM Tris-H₃PO₄ (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃ and 33% glycerol
Storage: Upon receipt store at -20°C

手册和实验方案

📝 User Guide: PageRuler Prestained Protein Ladder